DNA Contraction in Bio Organism

DNA library is a collection of one organisms DNA fragments that are stored with in a host organism. DNA construct is an artificially constructed segment of nucleic acid that is going to be transplanted into target cell DNA library; the long strands of chromosomal DNA are cut into thousands of fragments. Each fragment is stored and replicated in a separate host organism, such as a bacterium. It often contains DNA insert, which contains the gene sequence encoding a protein of interest that has been sub cloned into vector, which contains bacterial resistance genes for growth of bacteria and promoters for expression in the organism. For example for the construction of human DNA library, the long strands of chromosomal DNA are cut into thousands of pieces or fragments.

Each fragment is stores and replicated in separate host organisms such as bacterium .Bacteria can quickly replicate the DNA to produce detectable quantities. DNA construct may express wild type protein, prevent the expression of certain genes by expressing competitors or inhibitors or express mutant proteins, such as deleting mutations. DNA construct is often used inn molecular biology to analysis macromolecules such as proteins. When a gene is identified and copied, it is known as cloned. Genes are sequences of DNA code for the synthesis of one or more types of RNA molecules used to make proteins.